To validate the power of within-tumor tests, extreme tails analysis, and pooled tests, we demonstrated highly significant associations between increased expression and increased invasiveness, p = 2.3 × 10−45 for total Keratin 14 and p = 1.0 × 10−13 for Keratin 14 normalized to the imaged cross-sectional area. The gene discussed is KRT14; the disease is neoplasm.