In the present study, we examined the presence and distribution of new lymphatic vessels in glioma samples from seven patients via immunohistochemical staining for the neovascular endothelial cell marker molecule CD34, lymphatic endothelial hyaluronic acid receptor 1 (LYVE‐1), prospero‐related homeobox 1 (Prox1), the neuroepithelial stem cell protein nestin, and hypoxia‐inducible factor 1α (HIF‐1α). The gene discussed is LYVE1; the disease is central nervous system cancer.