Interestingly, we found that upregulation of FOXD2-AS1 significantly induced STAT3 reporter activity, while it had no effect on TCF/LEF or Notch reporter activity, which reflect the activation level of Wnt signaling and Notch signaling, two important signaling pathways in cancer stemness regulation (Fig. 5d, Supplementary Fig. 2a, b). This evidence concerns the gene STAT3 and cancer.