Here, we further found that although Nur77 2G (a Nur77 mutant that lost its DNA binding ability due to 2 Cys to Gly mutations in its zinc finger [17]) could still effectively inhibit HCC cell proliferation, the inhibitory effect of Nur77 2G was significantly impaired as compared with that of wild-type Nur77 (Supplementary Fig. 1a), implying that Nur77 may also directly regulate the transcription of its downstream target genes to suppress HCC cell proliferation. The gene discussed is NR4A1; the disease is hepatocellular carcinoma.