For example, in PD-L1-negative lung cancer and cholangiocarcinoma, HHLA2 was more prevalent than PD-L1; and HHLA2 overexpression was common.15 20 Therefore, this theory may overestimate the proportions of immunological ignorance and tolerance patterns, and inversely underestimate the proportion of adaptive immune resistance and intrinsic induction patterns. This evidence concerns the gene HHLA2 and cholangiocarcinoma.