To study the effect of ZIKV infection on the expression of CCN1 in mouse brain, the whole brain of mice was stripped and homogenized; RT-qPCR results showed that CCN1 expression at the mRNA levels was up-regulated in the ZIKV group on days 2 and 5 post-infection, compared with that of the mock group (Figure 2(d)). This evidence concerns the gene CCN1 and Zika virus infectious disease.