We next assessed the EMT‐related proteins, including matrix metalloproteinases (MMPs), E‐cadherin, and vimentin, which promote tumor cell invasion.26 As expected, western blotting in Figure 3E suggested that MMP 2, 9, and vimentin proteins expression were obviously decreased, nevertheless the E‐cadherin was upregulated after celastrol incubation. This evidence concerns the gene CDH1 and neoplasm.