EMT is an important molecular mechanism of invasion and metastasis of malignant tumors.34 A large amount of evidence from experimental and clinical researches have highlight the important role of EMT in CCA metastasis.38 Celastrol has been reported to exhibit inhibitory effects on the progression of EMT in various of cancers.39, 40, 41 Our present studies proved that celastrol substantially inhibited the levels of epithelial marker E‐cadherin and decreased the levels of mesenchymal markers N‐cadherin and vimentin. This evidence concerns the gene VIM and cancer.