Then we over expressed the three miRNAs in HCC cells and co-cultured with M2 macrophages; the results revealed that over expressed miR-149-5p could reverse co-culture's function to MMP9, both in SK-HEP-1 and HA22T cells (Fig. 3C-D), so was the invasion and migration capacity (Fig. 3E-F). The gene discussed is MMP9; the disease is hepatocellular carcinoma.