For the first time, we applied WGCNA analysis to different Gleason scores and different pathological T grades in prostate cancer and found the key genes AURKB, CCNB2, CCNA2, MAD2L1, BUB1B, CDK1, CCNB, KIF1, NCAPG, PBK, NUSAP1, TOP2A, MELK, KIF20A, and ASPM. This evidence concerns the gene TOP2A and prostate cancer.