For the first time, we applied WGCNA analysis to different Gleason scores and different pathological T grades in prostate cancer and found the key genes AURKB, CCNB2, CCNA2, MAD2L1, BUB1B, CDK1, CCNB, KIF1, NCAPG, PBK, NUSAP1, TOP2A, MELK, KIF20A, and ASPM. The gene discussed is PBK; the disease is prostate carcinoma.