With the advances in whole exome sequencing (WES) and whole genome sequencing (WGS) combining the other multi-omics studies, a large proportion of somatic mutations in CRC were identified, including TP53, APC, TTN, KRAS, PIK3CA, SMAD4, FBXW7 and RNF43, which drive the evolution of a malignant CRC4. The gene discussed is APC; the disease is colorectal carcinoma.