As shown in Fig. 5C,D, compared to nontreated controls, NEK2‐OE KMS11 or RPMI 8226 MM cell lines showed only a slight decrease in their capacity to form colonies, while those cells expressing BECN1‐shRNA showed a significant decrease in colony formation when treated with the same dose of BTZ, indicating that silence of Beclin‐1 prevents BTZ resistance in NEK2‐OE MM cell lines. This evidence concerns the gene CASC3 and Miyoshi myopathy.