In this study, the USP7 inhibitor significantly decreases Beclin‐1 in NEK2‐OE MM cells, and the decrease in Beclin‐1 mediated by USP7 inhibitor can be blocked by MG132, suggesting that USP7 is a novel Beclin‐1 regulator, which stabilizes Beclin‐1 through deubiquitination. Here, NEK2 is linked to Miyoshi myopathy.