Given that the mechanism by which CRP1-7 causes anti-SVCV neutralizing activity seems to promote an IFN-independent antiviral state, we proceeded to explore such observations in a homologous experimental system composed of the zebrafish-derived ZF4 cell line (also susceptible to SVCV infection), since the EPC cell line comes from fat-head minnow (Pimephales promelas), another fish species within the same family as the zebrafish (Cyprinidae)51. This evidence concerns the gene IFNA1 and infection.