RIPK3 and atherosclerosis: To try to understand how Ripk3 deletion in macrophages could lead to an increase in atherosclerosis severity, we isolated and cultured BMDMs from control and Ripk3ΔMΦ-Cre bone marrow and performed real-time quantitative PCR (qPCR) for a panel of genes that are related to necroptosis, inflammation, macrophage activation and endothelial cell activation (Fig. 6A and Fig. S7A).