We then evaluated the relevance of tumor and microenvironmental Gal-3 to breast cancer biology and performed an orthotopic injection of 4T1-scramble (SC) or 4T1-shRNA-Gal-3 (SH) cells in Lgals3+/+ (WT) or Lgals3−/− Balb/c female mice. Here, LGALS3 is linked to neoplasm.