Next, we determined the phosphorylation of EphB4 and its downstream adaptor protein Crk1 (MAPK14) in lysates from human A2780cp20 ovarian cancer cells by immunoprecipitation with anti-EphB4 and anti-Crk1 antibodies followed by immunoblotting with phospho-specific antibodies. This evidence concerns the gene EPHB4 and ovarian carcinoma.