The analysis was performed by comparing three polymorphisms located in the DRD2 gene—rs1076560, rs1799732 and rs1079597 (Taq1B) and personality traits measured with the Big Five Questionnaire (NEO FFI), as well as anxiety measured with the State Trait Anxiety Inventory (STAI) in two groups of subjects—e-cigarette users and the non-smoking/ non-vaping control group. This evidence concerns the gene DRD2 and Anxiety.