The blockage of FGF-signaling in BGJ398-treated GIST T-1R cells was confirmed by immunoblotting, which revealed the lack of phosphorylation of FGFR substrate 2 (FRS2) known as an adaptor protein that plays a critical role in FGF-signaling (Figure 4D). The gene discussed is FRS2; the disease is gastrointestinal stromal tumor.