To this end we co-cultured γδ T cells (untouched or untouched isolated and IL-2/IL-12/IL-18-stimulated, or TCR/IL-2/IL-12/IL-18-stimulated) with T24 (bladder carcinoma), MCF7 (breast cancer) or WM115 (melanoma) cancer cells for 96 h and then evaluated the cell cycle status of the tumor cells by EdU-incorporation assay. The gene discussed is IL2; the disease is melanoma.