ZIKV was able to establish a productive infection in microvascular endothelial (HMEC-1) cells (Figure 9A,B), since viral E protein was detected at high levels on the cell membrane surface mainly at 24 (45.62 ± 1.76%) and 72 h (38.22 ± 1.20%) PI time points, with a significance level of p < 0.0001 when compared to the mock HMEC-1 cells (22.9- and 19.2-fold higher, respectively). Here, PROS1 is linked to infection.