RNASE1 and infection: Different EVs stimulation assays were performed using naïve THP-1 cells in the presence of ZIKV (MOI 1), mock C6/36 EVs, or ZIKV C6/36 EVs (small and large isolates as the same for the RNase A + UV-treated and untreated samples) (Figure S5), which were evaluated for possible infection by means of ZIKV E protein detection by FACS (see Materials and Methods).