The same result is observed when we co-culture DEF-Exo induced from DTMUV infected DEF cells (relatively has low level of miR-148a-5p), DEF-Exo induced from PBS control group (relatively has high level of miR-148a-5p) with PBMC, These results suggest that the high level of TLR3 after DTMUV can both increase the expression of IFN-β and decrease the level of miR-148a-5p to resist DTMUV infection. This evidence concerns the gene IFNB1 and infection.