Since the luciferase activity of the lysate extracted from the PRKD3‐sgRNA‐1‐edited cells was higher than the one from the PRKD3‐sgRNA‐2‐edited cells, we decided to use PRKD3‐sgRNA‐1 to knockout PRKD3 gene in the two breast cancer cell lines. This evidence concerns the gene PRKD3 and breast carcinoma.