In IRONOUT HF, 225 patients with symptomatic heart failure with reduced ejection fraction and iron deficiency (defined as serum ferritin of less than 100 ng/mL or serum ferritin 100 to 300 ng/mL with a transferrin saturation of less than 20%) were randomly assigned to receive iron polysaccharide 150 mg twice daily for 16 weeks52. This evidence concerns the gene TF and hydrops fetalis.