In summary, in this study, we demonstrate that the decrease in the therapeutic effect of the Anchored GM-CSF vaccine combined with anti-PD-1 antibodies is related to the upregulation of Tim-3 expression on TILs and that Tim-3 blockade following the Anchored GM-CSF vaccine and anti-PD-1 antibody therapy can further overcome adaptive immune resistance in established PCa. The gene discussed is CSF2; the disease is posterior cortical atrophy.