To further explore the downstream target of circ_100395/miR-1228 axis in ovarian cancer, we predicted target genes of miR-1228 from TargetScan and miRanda databases, while p53 was found to be the potential downstream targets, as the predicted 3ʹUTR binding regions miR-1228 on p53 was shown in Figure 5A. To explore their relationship, we constructed the p53 mutant type 293T cells. This evidence concerns the gene TP53 and ovarian cancer.