In order to determine whether ANG also mediates the production of small RNA and whether the 3′-end fragment of 5S rRNA enhances stemness in other types of CSCs, we examined the effect of ANG in P19 embryonal carcinoma cells, a well-characterized cell line that has been shown to possess pluripotent CSC properties and to be able to differentiate into cell types of all three germ layers48. Here, ANG is linked to embryonal carcinoma.