In line with our previous work, bladder carcinoma T24 cells transduced with RAdncCD40L for 36 hours exhibited significant reduction in cell viability, while sCD40L (1 μg/ml, 36 h) treatment did not show any cell viability reduction (Fig. 1A) despite full sCD40L biological activity (Fig. 1B) evidenced by phosphorylation of JNK, PI3k/Akt and ERK kinases in addition to IKβα degradation, where sCD40L addition resulted in comparable levels of AKT and JNK phosphorylation to those transduced with RAdnCD40L at 20 min treatment. Here, CD40LG is linked to urinary bladder carcinoma.