AKT1 and urinary bladder carcinoma: In line with our previous work, bladder carcinoma T24 cells transduced with RAdncCD40L for 36 hours exhibited significant reduction in cell viability, while sCD40L (1 μg/ml, 36 h) treatment did not show any cell viability reduction (Fig. 1A) despite full sCD40L biological activity (Fig. 1B) evidenced by phosphorylation of JNK, PI3k/Akt and ERK kinases in addition to IKβα degradation, where sCD40L addition resulted in comparable levels of AKT and JNK phosphorylation to those transduced with RAdnCD40L at 20 min treatment.