ACTA2 and cancer: Differentially expressed genes across clusters in P13 (Fig. 6b, Supplementary Data File 4) revealed an ecosystem of cells including hepatocytes (ALB, FGG), cancer-associated fibroblasts (ACTA2, TAGL), endothelial (KDR, VWF), myeloid-derived (HLA-DQB1, CD68), and sporadic B-cells (IGJ, CD79A), which is consistent with the lack of immune infiltrate on histological examination in this patient (Supplementary Fig. 1b).