After permeabilization with a saponin pulse, the fluorescence intensity peak of GRA5 overlapped with the DBA fluorescence intensity peak and the maximum fluorescence intensity region of GRA5 was concentrated toward the left of the DBA fluorescence intensity region (Fig. 4B), indicating that GRA5 was localized toward the exterior of the DBA stain (cyst wall) and suggesting that GRA5 is a component of the cyst membrane (Fig. 4B). The gene discussed is RPS19; the disease is cyst.