KLF1 and thalassemia: Since screening by hematological indices may result in misdetection of carriers due to factors like co-inheritance of α-thalassemia, mutations in β- and δ-globin genes, mutations in KLF1 gene and iron deficiency anemia [15, 27, 28], this study applied a comparatively cheaper and easy-to-perform DNA-based HRM curve analysis method to confirm and characterize mutations in the β-globin gene.