Among 23 candidate genes, damaging DNVs in nine of them (ROCK2, CHD4, KDM5A, APBB1, USP4, PYGL, CAD, BOD1L1, and GANAB) were found in syndromic CHD patients, in three of them (HSP90AA1, IQGAP1, and TJP2) were found in isolated CHD patients, and remaining were found in either both types of CHD or those with unknown phenotype status (Additional file 5: Table S16). The gene discussed is USP4; the disease is coronary artery disorder.