The proteolytic cleavage of LC3 is considered a hallmark of autophagy, our analysis showed that in cells from HD patients, the fast-migrating bands, corresponding to the cleaved LC3-II form, is significantly reduced compared to its level in fibroblasts from control healthy donors (Figure 8B,C, Student t-test p-0.0341), suggesting that the autophagic flux has been compromised in HD cells. The gene discussed is MAP1LC3A; the disease is Huntington disease.