The gradual increase in rhabdomyosarcoma stemness could be further substantiated by the detected downregulation of 10 out of 18 genes that are associated with mesenchymal stem cell senescence [25] and by the upregulation of genes involved in early mesenchymal development and maintenance of myogenic precursors, i.e., SOX4 [45,46] and PITX2 [23]. Here, PITX2 is linked to rhabdomyosarcoma.