TNF and neoplasm: Ex vivo restimulation of the spleen and tumor draining lymph node (dLN) on day 7, when tumors were of equivalent sizes, revealed a reduction in anti-tumor T cell responses (as measured by CD4+ Th1 TNF-α+ and IFN-γ+ cells) in mice bearing p53-null tumors from either isogenic (Figures 3G, 3H, and S3H) or the GEMM-derived lines (Figures S3I and S3J).