MYO1B and cyst: To assess the impact of T. gondii infection as well as the importance of the exported effector proteins on the host transcriptome during chronic infection, we performed high-throughput RNA sequencing on mouse brains harvested 10–12 weeks p.i. RNA was prepared from the whole brain of uninfected, ME49-, ASP5-KO-, and MYR1-KO-infected mice previously tested for behavior and assessed for cyst load.