Besides, we performed western blot assay to check theapoptosis markers Bax and cleaved Caspase-3 expression in these cell populationand the results showed their significantly enhanced expression inOIP5-AS1-depleted C33A cells, which was consistent with Annexin V-FITC-PIapoptosis analysis (Figure 2G and H).Together, these data demonstrated that down-regulation of OIP5-AS1 could inhibitcell proliferation and promote cell apoptosis in cervical cancer cells. This evidence concerns the gene ANXA5 and cervical cancer.