To assess the effect of rTM on the inflammatory response in the local and distant organs during ischemic AKI, we measured the messenger RNA (mRNA) expression levels of inflammatory cytokines including tumor necrosis factor-α (TNF-α), keratinocyte-derived chemokine (KC), and IL-6 in the lung and kidney using quantitative polymerase chain reaction (PCR). The gene discussed is TNF; the disease is acute kidney injury.