To evaluate the functional impact of ETV5 levels in neuroblastoma cells with different mutant ALK backgrounds, we performed transient (siRNA) and stable (shRNA) ETV5 knockdown in CLB-GA (ALKR1275Q), NB-1 (ALKamp), SK-N-AS (ALKwt, NRASQ61K), and SH-SY5Y (ALKF1174L) cells. Here, ETV5 is linked to neuroblastoma.