Infection of plain Huh7-S10-3 liver cells with the genotype 3 HEV Kernow P6 virus yielded 2.5 ± 0.5 log10 FFU/ml infectious virus and 10,270 ± 1,144 HEV RNA copies/ml, while overexpression of RIG-I reduced the virus titer by 10-fold, i.e., to 1.5 ± 0.5 log10 FFU/ml and 3,202 ± 569 HEV RNA copies/ml. Here, RIGI is linked to infection.