Thus, to demonstrate the power of DENDRO and to better understand the relationship between ICB response and intratumor heterogeneity, we profiled the single-cell transcriptomes across three conditions derived from two melanoma cell lines (Fig. 3a): B16 melanoma cell line, which has shown modest initial response to ICB treatment but eventually grows out, and Res 499 melanoma cell line (R499), which was derived from a relapsed B16 tumor after combined treatment of radiation and anti-CTLA4 and is fully resistant to ICB [51]. This evidence concerns the gene CTLA4 and melanoma.