In dysferlinopathies, almost all fibers are stained with antibodies against dystrophin 1, 2, and 3, merosin, β-spectrin, and α, β, and γ sarcoglycans; but the muscle fibers are looked completely deficient against dysferlin antibody. Here, DYSF is linked to neuromuscular disease caused by qualitative or quantitative defects of dysferlin.