Based on the regulatory model (Additional file 1: Figure S1) and differential expression of mRNA between low and high aggressive HCC (see “Identification of gene differentially expressed between low and high aggressive HCC” section), the algorithm Iggy predicts the activation of complexes NFKB1::BCL3 and NFKB2::RELB and the deactivation of complex JUND::NACA. Here, NFKB1 is linked to hepatocellular carcinoma.