NPM1 and acute myeloid leukemia: Of note, the NPM1 and FLT3-ITD mutations seen in diagnostic blasts were found in only 2/95 (2%) and 1/57 (1.5%) individually plucked CFUs, suggesting that BM clonogenic myeloid progenitors from NPM1-mutated and NPM1/FLT3-ITD-mutated AML patients do not harbor either the NPM1 or the FLT3-ITD mutation present in the AML blasts (Figure 1D).