To further study the impact of MYXV virotherapy against malignant glioma, mouse glioma cell lines derived from C57BL/6J NPcis mice (Trp53+/−/Nfl+/−), which spontaneously developed high-grade gliomas and recapitulated many clinical phenotypes of the human disease, were tested in syngeneic C57BL/6J mice. This evidence concerns the gene NEFL and central nervous system cancer.