The fungi polysaccharide, Antrodan, a β-glucan isolated from A. cinnamomea mycelia, has shown its beneficial effects against NAFLD development by suppressing plasma MDA, GOT, GPT, total cholesterol, triglycerides, glucose, insulin, upregulating adiponectin, leptin, pAMPK, Sirt1, and downregulating PPARγ and SCEBP-1c, which apparently have covered a complete spectrum of therapeutic benefits to alleviate the liver injuries. The gene discussed is SIRT1; the disease is metabolic dysfunction-associated steatotic liver disease.