A high level of MUC1 expression (positive vs. negative: OR = 2.45, 95% CI = 1.38–4.35, P = 0.002) was associated with lymph node metastasis in patients with CRC than MUC2 expression (positive vs. negative: OR = 0.59, 95% CI = 0.47–0.73, P < 0.00001), but the association between MUC5AC expression and lymph node metastasis was not significant. This evidence concerns the gene MUC2 and metastatic malignant neoplasm in the lymph nodes.