A multiplex ELISA for 38 cytokines was performed (Figure 5d) identifying that MYC/Twist1-HCC cells had increased secretion of cytokines: Il13, Ccl2, Ccl5, Ccl7 and Cxcl1 (p<0.05; Fold change ≥2, mean ≥20 ng/ml) (Figure 5e, Figure 5—figure supplement 1b). The gene discussed is CXCL1; the disease is hepatocellular carcinoma.