Conditioned media from MYC/Twist1- but not MYC-HCC cells was able to elicit changes in the morphology of macrophages to resemble M2 phenotype (McWhorter et al., 2013) (Figure 5c) and increased expression of M2 markers: Cd206, Arg1 and Cc3cr1 (Figure 5c), but not M1 markers: iNos, Ccr2, Ifnar2 (Figure 5—figure supplement 1a). This evidence concerns the gene CCR2 and hepatocellular carcinoma.