Macrophage depletion with clodronate liposomes (Moreno, 2018) but not control liposomes, in mice orthotopically transplanted with MYC/Twist1-HCC had reduced intrahepatic (p=0.0006, FC 4.4) and lung metastases (p<0.0001, FC 8.8) (Figure 4e–f). This evidence concerns the gene TWIST1 and hepatocellular carcinoma.