IFNG and cancer: Co-culture experiments of P73-primed T cells with breast cancer cell lines showed a 2.6-fold borderline significant increase (p = 0.06) in IFN-γ release after incubation with A2 + /high in comparison to P73-primed cells incubated with A2 + /low (1.3-fold) or A2−/high (1.6-fold) cancer cells (Fig. 4c). The representative ELISpot image clearly shows the increase in IFN-γ spot forming units in the A2 + /high cancer cell/T cell co-culture but not in the other co-cultures.