Using different breast cancer cell lines, we demonstrated that LDHC41−55and LDHC288−303 specific T cells were capable of recognizing and eradicating HLA-A*0201 positive/LDHC positive tumor cells, while no specific cytolytic activity was detected against HLA-A*0201 negative/LDHC positive tumor cells. Here, LDHC is linked to breast carcinoma.