Incubation with A2 + /high breast cancer cells specifically increased IFN-γ secretion (4.5-fold, ns) of P11-primed T cells (Fig. 3c) in comparison to T cell responses generated against A2−/high or A2 + /low breast cancer cells, confirming HLA-A*0201 restriction and suggesting the existence of a threshold for LDHC expression to elicit an immune reaction. This evidence concerns the gene HLA-A and breast carcinoma.