However, in accordance with Fig. 3a, the abundance of p62-positive pneumococci containing-vacuoles containing cells was not changed until 120 min p.i. (Fig. 4c), indicating that NDP52-delocalized PcLV was induced after 90 min of infection and continued until 120 min p.i. (Supplementary Fig. 6B) in FIP200 KO MEFs. This evidence concerns the gene CALCOCO2 and infection.