To evaluated the role of AKT signal pathway on cell invasion in NSCLC, H1975 cell lines were subjected to treatment with DMSO, N-Shh (0.5 mg/mL), AKT inhibitor MK-2206 2HCI (1umol/L), AKT inhibitor MK-2206 2HCI(1umol/L) for 30 min, then administrated N-Shh (0.5 mg/mL) for 24 h (Fig. 5a & b). The gene discussed is AKT1; the disease is non-small cell lung carcinoma.