Although many cancers activate the pathway either by activation of smoothened or inactivation of patched, some tumors involve in SHH signaling by increasing the expression levels of GLI, such as rhabdomyosarcoma, osteosarcoma, glioma, breast cancer, pericytoma, prostate cancer, and Ewing sarcoma family of tumors. This evidence concerns the gene GLI1 and Ewing sarcoma.