To examined whether RhoA/ROCKs and Rac1/PAK influenced HIF-1α in HCC, the MHCC97H cells were treated with exoenzyme C3 (800 ng/ml, RhoA inhibitor), Y27632 (50 μM, ROCK inhibitor), NSC23766 (50 μM, Rac1 inhibitor) or IPA-3 (10 μM, PAK inhibitor) in hypoxia. The gene discussed is RAC1; the disease is hepatocellular carcinoma.